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Test of antithrombosis properties of SSCMC in vitro and in vivo . a) Surgical schematic of in vitro blood circulation in rabbits. b) Diagrams of blood cells adhering to the inner lumen of both catheters before and after modification of in vitro blood circulation for 2 h. c) Comparison of blood cells adhering to the surfaces of both catheters before and after modification of the inner lumen of both catheters under SEM. d) ∼ f) Quantification of thrombus weight, lumen occlusion and blood flow rate formed in the lumen of the two catheters, respectively. g) Blood circulation modeling to perform blood correlation analysis. h) Thrombin-antithrombin III enzyme complex (TAT) (an early marker of coagulation activation). i) Tissue-type fibrinogen activator-inhibitor 1 complex (PIC) (risk indicator for venous thromboembolism). j) Thrombomodulin (TM). k) Platelets (PLT). l) White blood cells (WBC). m) Serum albumin (ALB). n) Acute inflammation indicator c-reactive protein (CRP). o) Tumor necrosis factor (TNF-α). p) IL-6. q) IL-10. r) Liver function indicator alanine aminotransferase (ALT). s) Kidney function indicator serum creatinine (Scr). Error bar represents the mean ± SD. Significance was calculated by one-way analysis of variance. ∗∗∗∗ p < 0.0001. N = 4, averaged.

Journal: Materials Today Bio

Article Title: Robust and ultra-stable nanohesive-based solid-like slippery coating under dynamic blood flow environment for durable prevention of thrombosis and biofouling

doi: 10.1016/j.mtbio.2025.102449

Figure Lengend Snippet: Test of antithrombosis properties of SSCMC in vitro and in vivo . a) Surgical schematic of in vitro blood circulation in rabbits. b) Diagrams of blood cells adhering to the inner lumen of both catheters before and after modification of in vitro blood circulation for 2 h. c) Comparison of blood cells adhering to the surfaces of both catheters before and after modification of the inner lumen of both catheters under SEM. d) ∼ f) Quantification of thrombus weight, lumen occlusion and blood flow rate formed in the lumen of the two catheters, respectively. g) Blood circulation modeling to perform blood correlation analysis. h) Thrombin-antithrombin III enzyme complex (TAT) (an early marker of coagulation activation). i) Tissue-type fibrinogen activator-inhibitor 1 complex (PIC) (risk indicator for venous thromboembolism). j) Thrombomodulin (TM). k) Platelets (PLT). l) White blood cells (WBC). m) Serum albumin (ALB). n) Acute inflammation indicator c-reactive protein (CRP). o) Tumor necrosis factor (TNF-α). p) IL-6. q) IL-10. r) Liver function indicator alanine aminotransferase (ALT). s) Kidney function indicator serum creatinine (Scr). Error bar represents the mean ± SD. Significance was calculated by one-way analysis of variance. ∗∗∗∗ p < 0.0001. N = 4, averaged.

Article Snippet: At predetermined time intervals (after 0, 5, 30, and 60 min of circulation), rabbit blood was collected for biochemical analysis, including thrombin-antithrombin complex (TAT, CUSABIO), fibrinolytic-α2 fibrinolytic inhibitor complex (PIC, mlbio), thrombomodulin (TM, mlbio), platelet (PLT), white blood cell (WBC), serum albumin (ALB, proteintech), C-reactive protein (CRP, proteintech), tumor necrosis factor-α (TNF-α, proteintech), and inflammatory and immunosuppressive factors (IL-6, IL-10, proteintech) to assess physiological parameters such as coagulation, inflammatory response, and organ function.

Techniques: In Vitro, In Vivo, Modification, Comparison, Marker, Coagulation, Activation Assay